A. Kitzis, L. Tichonicky, N. Defer
Apr 14, 1980
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Influential Citations
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Quality indicators
Journal
Biochemical and biophysical research communications
Abstract
Abstract Sodium butyrate is able to modify gene expression. It was then interesting to study its effect on chromatin structure. Butyrate modified the accessibility of chromatin from HTC cells to micrococcal nuclease : chromatin was digested to a higher rate until 1.5 % of the DNA was rendered acid soluble, in longer digestion it was attacked to a lesser extent than chromatin from untreated cells. When digestion was prolonged, both chromatins were equally digested. The released fragments were submitted to sucrose gradient analysis. The results confirmed the previous observations, in addition it showed that the mono- and dimers of nucleosomes preferentially originated from butyrate-treated cells, while the larger fragments were released from untreated cells. The increase in accessibility of a small part of chromatin induced by butyrate could be correlated with the effect of this compound on gene expression.