Guo-Sheng Xiang
2011
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Quality indicators
Journal
Journal of Soochow University
Abstract
Objective To explore the effect of sodium metavanadate on cell apoptosis in HL-60 cells,and preliminarily judge whether the impact associate with ppGalNacT2.Methods MTT was used to observe cell proliferation.DNA(deoxyribonucleic acid) line was detected by agarose gel electrophresis.Changes of cell nucleus stained by Hoechst 33258 in HL-60 cells treated by sodium metavanadate were studied by fluorescence microscope.Flow cytometry(FCM) was used to detect cell apoptosis.Molecular docking and molecular dynamics simulations were used to judge the relationship between vanadium and the activity of ppGalNac-T2.Results Different concentrations of sodium metavanadate were used to treat HL-60 cells;24,48,72 h later MTT results showed that low doses of sodium metavanadated promoted cell proliferation,high doses of sodium metavanadate inhibited cell growth.DNA fragment appeared after HL-60 cells were exposed to high concentrations of sodium metavanadate,and cell nucleus were concentrated and fragmented.Treated with different concentrations of sodium metavanadate in concentration of 5×10-7,1×10-7,1×10-8,1×10-9,1×10-11mol/L,the apoptotic rate was(69.1±2.3)%,(56.3±4.9)%,(39.6±6.5)%,(31.4±1.6)%,(12.2±3.4)%.The apoptotic rate in the negative control group was only(1.2±0.5)%(P0.05).VO3 might affect the activity of the enzyme by stemming conformational changes of a key loop of ppGalNAcT2.Conclusion Sodium metavanadate can induce apoptosis in HL-60 cells,which may associate with the activity of ppGalNac-T2.