Yan Shaochun
2008
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Quality indicators
Journal
Journal of Baotou Medical College
Abstract
Objective : To study the effect of sodium orthovanadate on the expression of cell cycle protein Cyclin D1 in mouse NIH3T3 cell line.Methods: Mouse NIH3T3 cells grown in cell culture were treated with sodium orthovanadate at doses of 0,0.1,1.0,5.0,10.0,100.0,200.0 μmol/L respectively for 4 hours and the cell extracts were subjected to Western blotting technique to examine the expression of cyclin D1.Results: After application for 4 hours,as compared with the control,sodium orthovanadate at the dose of 5.0 or 10.0 μmol/L induces the expression of Cyclin D1 significantly whereas at the dose of 100.0 or 200.0 μmol/L,it causes the reduction of cyclin D1 expression.Conclusion: Sodium orthovanadate may affect the growth of NIH3T3 cells through the bi-directional control of cyclin D1 expression including both down-regulation and up-regulation.