F. Amalric, M. Nicoloso, J. Zalta
Jan 19, 1974
Citations
0
Influential Citations
2
Citations
Journal
Biochimica et Biophysica Acta
Abstract
Abstract Sodium p -aminosalicylate , a deproteinising agent used in the course of DNA extraction, interacts with DNA. This interaction results in obtaining a definite part of DNA in a single-stranded form. This denaturation process can be achieved by freezing the DNA solution below −12 °C in presence of sodium p -aminosalicylate . The amount of the singlestranded fraction depends on the sodium p -aminosalicylate concentration and the number of freezing cycles. Hybridisation experiments show that this denaturation process is not random and that DNA in solution or within chromatin is sensitive to the same extent. The role of these definite destabilized DNA sequences in the structure of chromatin is discussed.