A. C. Kaelin
Nov 1, 1975
Citations
0
Influential Citations
8
Citations
Quality indicators
Journal
British Journal of Haematology
Abstract
Chemical treatment of human factor VIII by sodium periodate resulted in increased levels of procoagulant activity when measured by the two‐stage assay. Increased levels of factor‐VIII activity were observed following periodate treatment of factor‐VIII concentrates and normal, haemophilic and von Willebrand's disease plasmas. However, sodium periodate treatment of haemophilic plasmas which were completely deficient in factor‐VIII activity did not cause any reduction in the clotting times of two‐stage assays. No increase in procoagulant activity following oxidation of factor VIII could be detected by the one‐stage assay method. Gel filtration of factor‐VIII concentrates before periodate treatment showed that the protein exhibiting factor‐VIII activity had an apparent molecular weight in excess of 1 000 000, whilst after periodate treatment the apparent molecular weight was reduced to 690 000.