A. Keith, A. Waggoner, O. Griffith
Nov 1, 1968
Citations
0
Influential Citations
52
Citations
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abstract
the stearate spin label: The 12-nitroxide methyl stearate (I) was prepared from 12-keto methyl stearate by the procedure of Keana, Keana, and Beetham4 for converting ketones into stable nitroxide free radicals. The details of this synthesis are described elsewhere.5 fatty acids (palrnitic, stearic, oleic, linoleic, and linolenic acids), known neutral lipids (olive oil glycerides), and mixtures of all three classes of lipids. In an additional control experiment, mixtures were made of the methyl ester nitroxide with whole Neurospora phospholipids, with whole Neurospora free fatty acids, with whole Neurospora neutral lipids, and with the three lipid fractions combined. Each of these mixtures was then separated by this thin-layer technique. In all cases the nitroxide migrated as a neutral lipid.