Guanzhuo Wang, Kanyi Pu, Xinhai Zhang
Sep 26, 2011
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Influential Citations
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Chemistry of Materials
Abstract
A star-shaped glycosylated conjugated oligomer, 4,4′,4″-tris(4-(2-(4-(benzo[d]thiazol-2-yl)phenyl)-9,9′-bis(6-thiol-β-d-glucose)-hexyl)-fluoren-7-yl)phenylamine (TFBS), is synthesized for two-photon fluorescence imaging of live cells. The high density of hydrophilic sugar side groups induces self-assembly of TFBS into nanoparticles in water with an average diameter of 61 nm. Because of the self-assembled nanostructure, TFBS has a higher quantum yield in water (Φ = 0.10), compared to its cationic counterpart, 4,4′,4″-tris(4-(2-(4-(benzo[d]thiazol-2-yl)phenyl)-9,9′-bis(6-N,N,N-trimethylammonium)-hexyl)-fluoren-7-yl)phenylamine (TFBC) (Φ = 0.03). In addition, TFBS has a large TPA cross section (δmax) of ∼1200 GM at 740 nm in aqueous media, which is significantly higher than that for TFBC. TFBS can be effectively internalized by the human cervical cancer cell line and accumulates in the cytoplasm, allowing for live cell two-photon fluorescence imaging upon 800-nm excitation. TFBS has also shown low cytotoxici...