G. Dubois, B. Zalc, F. L. Le Saux
Mar 1, 1980
Citations
0
Influential Citations
46
Citations
Journal
Analytical biochemistry
Abstract
Abstract A convenient method for large scale preparation of stearoyl[1- 14 C]sulfogalactosylsphingosine has been developed. The first step consists in preparing the lysosulfatide intermediate with a good yield, which we have successfully performed. In the second step, the lysoderivative is coupled to [1- 14 C]stearic acid through the acyl chloride procedure. The labeled substrate thus synthetized appears to be particularly convenient for cerebroside sulfatase determination, because of the stability of the 14 C isotope, compared to the other isotopes (tritium or 35 S) which have been previously used for the same purpose.