Yu. Yu. Nevmerzhitskaya, O. Timofeeva, A. Mikhaylov
Sep 1, 2013
Citations
1
Influential Citations
7
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Journal
Doklady Biological Sciences
Abstract
287 One of the main tasks of modern agricultural scii ence is the identification and development of new plant growth regulators that are environmentally safe, multifunctional, physiologically active compounds with both growthhregulating and antiistress properr ties. Steviol (133hydroxykaurr166enn188oic acid), the aglycon of diterpene glycosides of the plant Stevia rebaudiana Bertoni, is of particular interest [1]. In the literature, there is evidence that derivatives of steviol glycosides show gibberellinnlike activity [2]. The use of plant growth and development regulators that reduce the toxicity of pollutants and the accumulations of pollutants in plants is very important under the condii tions of increasing heavy metal pollution of agricull tural lands. In this connection, the goal of this work was to determine the protective effect of the giberellinn like diterpenoid glycoside stevioside on winter wheat under the influence of low temperatures and heavy metals. The results of the research have demonstrated that the diterpene glycoside stevioside (10 –8 M) increases the cold resistance of winter wheat to a higher extent in comparison with its derivatives obtained by chemii cal means and reduces the effects of cadmium and zinc on plant growth and changes of lectin activity. We studied the roots of seedlings of the winter wheat (Triticum aestivum L.) cultivar Mironovskaya 808. The studied compounds were synthesized in the Arbuzov Institute of Organic and Physical Chemistry of the Kazan Scientific Center of the Russian Acadd emy of Sciences (Kazan). Plants were grown under laboratory conditions in cuvettes with tap water at a light intensity of 100 W/m 2 and a 122h photoperiod at 23°C for nine days. The treated plants were grown on a stevioside (10 –8 M) solution. Then, fiveday old plants were transferred to heavy metals solutions of CdSO 4 and ZnSO 4 at concentrations of 10 µM and 1 mM. The concentrations of stevioside and heavy metals were selected in preliminary experiments. Soll uble lectins were extracted with 0.05 N HC1, celllwall lectins were extracted with 0.05% Triton XX100. The lectin activity was estimated by hemagglutination with erythrocytes of blood group O [3]. Protein content was determined accordingly to Bradford [4]. Cold resis tance was tested based on electrolytes leakage [5]. All experiments were performed in three biological replicates. The results shown in the figures and tables are arithmetic means and their standard errors. At the first stage of the research, we identified the compound with the highest antistress activity in …