E. Ohtsuka, S. Koide
Jun 6, 1969
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Journal
Biochemical and biophysical research communications
Abstract
Abstract Cortisol, 21-dehydrocortisol and cortisol 21-phosphate were added in vitro to isolated intact, and to fragmented liver nuclei obtained from adrenalectomized rats and the DNA-dependent RNA polymerase activities were measured. 21-dehydrocortisol possessed the greatest capacity to stimulate RNA synthesis of fragmented rat liver nuclei. It was effective at a concentration of 10−8 M which was comparable to the rate stimulated by cortisol at 10−4 M. The results suggest that the stimulation of RNA synthesis in rat liver nuclei by cortisol may be dependent upon its transformation to 21-dehydrocortisol in trace amounts and that this metabolite mediates the action.