D. Brash, R. Reddel, M. Quanrud
May 1, 1987
Citations
3
Influential Citations
98
Citations
Quality indicators
Journal
Molecular and Cellular Biology
Abstract
Strontium ion formed DNA-phosphate precipitates analogous to those formed by calcium but lacking the lethal and differentiation-inducing effects of calcium on many epithelial cell types in primary culture. Human primary bronchial epithelial cells were transiently and stably transfected by using strontium phosphate; the frequency of stable transformation with a plasmid carrying the simian virus 40 large-T-antigen gene was greater than 10(-4).