R. Paul, C. Ratledge
Aug 17, 1973
Citations
0
Influential Citations
2
Citations
Journal
Biochimica et biophysica acta
Abstract
Abstract Antranilate N-acetlytransferase, which is a constitutive enzyme, is responsible for the formation of N-acetylanthranilic acid which accumulated int he culture medium of certain mutants of Aerobacter aerogenes. It has been shown to be dissimilar to serine O-acetyltrasferase and not to be involved in the acetylation of a variety of aliphatic compounds. Aniline and m-aminobenzoic acid are, however, readily acetylated, the Km for the latter compound being the same as that for anthranilic acid, 13 mM. p-Aminobenzoic acid is only slowly acetylated and salicylic acid only acted as an inhibitor of the reaction. N-[ 3 H ] Acetyl [1,7- 14 C 2 ] anthranili acid was prepared but could not be shown to be deacylated for further metabolized when administered to any whole cell, cell extract or toluene-lysed cell preparation.