S. Imai, H. Seto, T. Sasaki
May 1, 1985
Citations
1
Influential Citations
40
Citations
Journal
The Journal of antibiotics
Abstract
Sir : Bialaphos (BA) is a metabolite produced by Streptomyces hygroscopicus SF-12932,3)and is now being in use as a herbicide. Based on the structures of biosynthetic intermediates such as phosphinic or phosphonic acid derivatives, which were produced by blocked mutants4,5), by the addition of monofluoroacetic acid1), or accumulated under fermentation conditions minus cobalt ion6), its biosynthesis has been assumed to proceed as follows; phosphoenolpyruvate-> phosphinopyruvic acid (formerly called a-keto MP-103) -> 2-phosphinomethylmalate -> deamino a-keto demethylphosphinothricin (formerly called a-keto MP-101)--demethylphosphinothricin (DMPT, formerly called MP-101)-> demethylbialaphos (DMBA, formerly called MP-102)->BA. Recently we have succeeded by mutagenesis with nitrosoguanidine in the isolation of three mutants (NP-51, NP-45 and NP-8) with blocks after DMPT (Fig. 1). We wish to report herein their accumulation products and abilities as converters. These data provide detailed information about the late steps in the biosynthetic