N. Izumiya, M. Muraoka, H. Aoyagi
Dec 1, 1971
Citations
0
Influential Citations
31
Citations
Journal
Bulletin of the Chemical Society of Japan
Abstract
Several tripeptides, H-Gly-DL-Ala-B-OH, in which B residues are DL-alanine, L-valine, L-leucine, L-phenyl-alanine, L-proline, and L-serine have been synthesized and the separation of each glycyltripeptide diastereomers by an amino acid analyzer have been studied. Among six peptides studied, diastereomeric mixtures of H-Gly-DL-Ala-L-Val-OH and H-Gly-DL-Ala-L-Leu-OH were separated completely. A procedure to determine the amounts of LL and DL diastereomer of H-Gly-Ala-Leu-OH by the use of the analyzer was developed, and this procedure was applied to examine the influence of coupling reagents for racemization during coupling of benzyl-oxycarbonyl-glycyl-L-alanine and L-leucine benzyl ester by several reagents.