D. Kipnis, C. Cori
Feb 1, 1957
Citations
0
Influential Citations
155
Citations
Quality indicators
Journal
The Journal of biological chemistry
Abstract
The isolated rat diaphragm has been used for the study of metabolic problems which involve the penetration as well as the utilization of sugars. Pen&ration alone can be studied by determining the intracellular and extracellular concentrations of sugars which are not appreciably metabolized. In this work, n-xylose was selected for this purpose. An analysis of the factors which have to be controlled in order to make the diaphragm a suitable test object for permeability studies of muscle has led to the conclusion that the usual diaphragm preparation has a number of serious disadvant.ages. A diaphragm preparation is described in which, in contrast to t.he usual preparation, none of the muscle fibers are cut. This preparation was found t,o be similar to the diaphragm in the intact animal wit,h respect to total tissue water, intracellular and extracellular spaces, rate of pent,ose penetration, and insulin responsiveness. The effects of a variet.y of parameters on pentose penetration in the presence and absence of insulin were also studied wit,h both kinds of diaphragm preparations.