Qian Li, Xinlei Tang, Jianghao Xu
Oct 12, 2021
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Influential Citations
7
Citations
Journal
Life sciences
Abstract
AIMS Studies had shown that stachyose had the function of enhancing immunity, However, the effect on food allergy of stachyose has not been researched. MAIN METHODS The egg allergen ovalbumin (OVA) was used to establish a food allergy model of BALB/c mice, and different doses of stachyose were given to mice in process. Using an enzyme-linked immunosorbent assay (ELISA) to assess the levels of IgE, IgG1, histamine and cytolines. Reverse Transcription-Polymerase Chain Reaction (RT-qPCR) was used to detect Gata-3, T-bet, TLR2, Myd88 and NF-κB. Western Blot was used to detect TLR2, NF-κB and p-IκB-α. Hematoxylin-eosin (HE) staining was used to detect intestinal morphology. KEY FINDINGS Compared with control group (OVA), serum IgE and IgG1 levels in the low-dose (1 mg/mouse) group and high-dose (5 mg/mouse) group of stachyose were significantly reduced (P < 0.05); the level of histamine was also decreased significantly (P < 0.05) and the body temperature decrease and allergic clinical features were alleviated after stachyose treatment. Furthermore, the level of interferon-γ (TH1 cytokine) was increased significantly (P < 0.05), whereas interleukin-4 (TH2 cytokine) was decreased significantly (P < 0.05), and the mRNA level of T-bet was increased. Furthermore, the expression of TLR2 and NF-κB were increased significantly (P < 0.05) in both transcription and translation levels after stachyose treatment. SIGNIFICANCE Food allergy was alleviated through improving TH1/TH2 balance by activating TLR2/NF-κB signal by stachyose.