Ji Yu-bin
2007
Citations
0
Influential Citations
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Citations
Quality indicators
Journal
Journal of Harbin University of Commerce
Abstract
To study the inhibitory effect of isohumulone in Humulus Lupulus L.on SGC-7901 and HepG-2,antitumor experiments in vitro,and reveal its mechanism.MTT assay was used to detect the cytotoxic effect of isohumulone on SGC-7901 and HepG-2.Influences of isohumulone on apoptosis were observed with flow cytometry(FCM).These cells underwent incubation with Rhodamine 123,followed by flow cytometry(FCM) for the measurement of mitochondrial membrane potential(MMP).IC50 of isohumulone to SGC-7901 is 13.4 μg/mL and to HepG-2 is 11.58 μg/mL.This paper observed the appearance of apoptosis peak after SGC-7901 and HepG-2 disposed by isohumulone for 48 hours.Isohumulone obviously reduced mitochondrial transmembrane potential(MMP) of SGC-7901 and HepG-2 in a dose dependence manner.The results showed that isohumulone had strong inhibitory action to both SGC-7901 and HepG-2 by inducing the apoptosis of tumour cells.Isohumulone induce the apoptosis through mitochondria-dependent pathway in SGC-7901 and HepG-2 cells,and the mitochondrial dysfunction is directly responsible for the pro-apoptotic effects of isohumulone.