L. Ainsworth, A. L. Carter, R. Common
1962
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0
Influential Citations
19
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Quality indicators
Journal
Canadian journal of biochemistry and physiology
Abstract
Estrone-16-C14 was administered intravenously to a laying hen. The urine excreted during the succeeding 24 hours contained, on the basis of chromatographic evidence, the following radioactive conversion products of estrone: estriol, 16-epiestriol, 17-epiestriol, 16-oxoestradiol-17 β, estradiol-17 β, and 16-oxoestrone. Three other minor conversion products were not identified. 16-Oxoestradiol and 16-oxoestrone were characterized more fully by chromatographic examination of their sodium borohydride reduction products.In a second experiment, urine and feces excreted during the first two 24-hour periods after administration of estrone-16-C14 were examined separately for radioactive conversion products. Of the radioactivity injected, 24.5% was recovered in the estrogen-containing extracts of the excreta. The urinary extracts contained 69% of the recovered radioactivity and the fecal extracts contained 31%.In a third experiment, recoveries of urinary metabolites after enzymatic and after acidic hydrolysis were ...