L. Cai
2013
Citations
0
Influential Citations
2
Citations
Journal
Journal of China Agricultural University
Abstract
This purpose of this research is to study the tissue residues of the tilmicosin phosphate in swine and determine the withdrawal time.A high-performance liquid chromatography(HPLC)method with detection at 280nm was used and validated for the determination of 10%tilmicosin phosphate soluble powder residues in pig tissues.The mobile phase was composed of acetonitrile,tetrahydrofuran,hosphate buffer and water with the volume ratio of 10∶5∶5∶80.The limits of detection(LOD)was 0.025μg/g,and the limits of quantitation(LOQ)was 0.05μg/g for liver and kidney;theLODwas 0.01μg/g and the LOQ was 0.02μg/g for muscle,fat and skin.Average recoveries ranged from 77.56% to 97.82%,coefficient of variance ranged from 0.78%to 10.5%.Administrated over 7-day by 80mg/L of Tilmicosin Phosphate in drinking water,the residue levels were higher in liver and kidney,and lower in muscle,fat and skin.According to the calculation by software WT1.4,the withdrawal period of tilmicosin residues is 5.73din liver,3.75din kidney,11.57din muscle,10.91din skin and fat.It is concluded that the expected withdrawal period of tilmicosin phosphate for swine should be 12days.