Wang Ke-min
2012
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Influential Citations
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Quality indicators
Journal
Chinese clinical oncology
Abstract
Objective To investigate the effects of neogambogic acid on proliferation and apoptosis of human breast cancer cell line MCF-7 and its mechanism.Methods MCF-7 cells were treated with different concentrations of neogambogic acid(0.5-20μg/ml) for 72 hours,and cell proliferation inhibition rate was detected by methyl thiazolyl tetrazolium(MTT) testing.Cell apoptosis was tested by flow cytometry(FCM) method with Annexin V-FITC/PI double staining.Mitochondrial membrane potential changes were detected by JC-1 test.Expression levels of Fas,FasL,caspase-3,caspase-8,caspase-9,Bax and Bcl-2 proteins were detected by Western blotting method.Results The proliferation of MCF-7 cells were inhibited in a dose-dependent manner when they were treated with 0.5-20 μg/ml neogambogic acid for 72h and IC50 was 1.763 μg/ml.0.5-3.0 μg/ml neogambogic acid could induce MCF-7 cells apoptosis in a dose-and time-dependent manner.Early apoptosis rate and total apoptosis rate of MCF-7 cells treated with 0.5μg/ml neogambogic acid for 48h were 3.7% and 7.2%,and those were 6.7% and 13.7% for 72h.Early apoptosis rate and total apoptosis rate of MCF-7 cells treated with 3μg/ml neogambogic acid for 48h were 69.5% and 71.7%,and those were 76.9% and 81.5% for 72h.Neogambogic acid(0.5,1.0,1.5 μg/ml) was effective to increase the proportion of mitochondrial transmembrane potential damaged cells.Apoptosis related protein FasL,caspase-3,caspase-8 and caspase-9 expression levels were increased in a dose-dependent manner.Fas and Bax protein levels changed little.The expression level of antiapoptotic protein Bcl-2 was decreased in a dose-dependent manner.Conclusion Neogambogic acid can inhibit cell proliferation of human breast cancer cell line MCF-7 by inducing cell apoptosis,and the mechanism may relate to death receptor and mitochondrial pathway.