Yun Wang
Mar 11, 2014
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Journal
Journal of Liquid Chromatography & Related Technologies
Abstract
N-butyl 3,5-di-(O-p-chlorobenzoyl)-D-2-deoxy-ribofuranoside is a very important medical intermediate, which is commonly constituted by two diastereomers (α- and β-anomers). In this paper the HPLC determination condition was studied for the first time. The Hypersil - SiO2(4.6 × 250 mm, 5 µm) column was used and the mobile phase was a mixture of hexane- isopropanol (98:2). The wavelength of determination was 240 nm. N-butyl 3,5-di-(O-p-chlorobenzoyl)-D-2-deoxy-ribofuranoside's diastereomers have been separated thoroughly with the HPLC determination condition. This method is accurate, simple, rapid, and suitable for laboratory and industry.