Jiang Shan-xiang
2011
Citations
0
Influential Citations
0
Citations
Journal
Chinese Veterinary Science
Abstract
To establish a simple assay method for dicyclanil by HPLC and to study the pharmacokine-tics characteristics of dicyclanil emulsion in healthy swine,dicyclanil emulsion were applied at a single dosage of 45 mg/kg to six swine.The plasma samples were treated by acetonitrile,and HPLC method was used to detect the dicyclanil concentration in plasma.In the experimental conditions,dicyclanil have a good linear relationship when concentration was from 0.05 to 20 μg/mL(r2=0.999),and the detect limit was 0.01 μg/mL.The dates were calculated based on 3P97 pharmacokinetics software.The pharmacokinetics of dicyclanil in swine was fitted to two-compartment with first order absorption open model,the main pharmacokinetics parameters were as follows:t1/2ka=9.836 h±0.198 h,t1/2α=13.649 h±0.124 h,t1/2β=20.745 h±1.187 h,tmax=17.576 h±0.216 h,Cmax=9.129 μg/mL±0.313 μg/mL,AUC=450.010 μg/mL·h±2.993 μg/mL·h.Results showed that the HPLC method was proprietary,accurate and sensitive,and it is sui-table for pharmacokinetic studies.Dicyclanil was absorbed and eliminated slowly in swine,with higher concentration in plasma.