Li Jie
2010
Citations
0
Influential Citations
0
Citations
Journal
Journal of Isotopes
Abstract
The 13C,15N2 doubly labeled Urea and 15N2 labeled Hydrazine hydrate were used as raw materials,and 13C,15N3-SEM·HCl was synthesized by a direct one-step synthesis which based on the reflux reacting.The reaction conditions of 13C,15N3 labeled semicarbazide hydrochloride were mainly investigated by the experiment designs,like single-factor design and orthogonal design.The following optimized conditions were obtained as follow: the mole ratio 15N2-N2H4 to 13C,15N2-urea was 1.4∶1,the reaction temperature was 135 ℃,the reaction time was 4.5 h,and the yield of 13C,15N3 labeled semicarbazide hydrochloride was above 90%,the purity ≥98%,the abundance of 13C ≥97%,the abundance of 15N ≥99%.This technology had many advantages,like short production cycle,high yield and sample later-processing.