L. Ying-hua
2006
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Journal
Chinese Journal of Industrial Medicine
Abstract
Objective To study the oral subchronic toxic effects of 85% N′-(2,4-dimethylphenyl)-N′-methylformamidine hydrochloride(DMAH)in rats.Method Four groups of rats were fed with foodstuff containing 85% DMAH in the doses of 625,125,25 and 0 mg/kg respectively for 3 months,observing general behavior,body weight,food consumption etc.At the end of experimental period,blood samples were collected for routine and biochemical assays,meanwhile,the 8 h urine samples were collected for routine and bio-chemical assays;the internal organs were also taken for calculating their organ coefficients and doing histopathological examinations.Result During experimental period,no obvious abnormality could be found in the low dose and control groups,but there were some symptoms,such as diminished activity,fidget,bloody secretion from nose and mouth,defilement round perineal area,damaged or ulcerated skin,reduction of body weight and food consumption,were observed in median and high dose groups.At the end of the experiment,the blood urea nitrogen(BUN),alkaline phosphatase(ALP)and aspartate aminotransferase(AST,only in males)were obviously risen in the rats of high dose group(P0.01 or0.05),and the total protein(TP),albumin(ALB),glucose(GLU),cholinesterase(ChE,only in females)were lowered in the median and high dose groups(P0.05).The urine protein showed positive results in some animals of median and high dose groups.The organ coefficients such as brain,heart,liver,kidney,adrenal gland and testes in high dose group and in medium dose group(only brain,kidney for female rats,and only brain,liver,testes for male rats)were higher than that of controls.Pathological examination showed that in high dose group,the circular vacuole,swelling and compressed hepatic sinusoid of liver cells in some animals were found;while in median dose group,only little circular vacuoles of peripheral liver cells could be seen.Conclusion 85% original DMAH could affect animal appetite,nutrition condition and behavior,decrease anti-infection potential,reduce body-weight and food coefficient,induce abnormality of biochemical indices(TP、ALB、GLU、BUN、ALP、ChE),organ coefficients and liver damage in some rats.The 90 day oral no-observed adverse effect levels(NOAEL)of 85% DMAH in rats were 2.34 mg/kg for female rats,and 1.59 mg/kg for male rats.