Zuo Yu
2007
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Quality indicators
Journal
China Tropical Medicine
Abstract
Objective To observe the effects of fosinopril,an angiotensin-converting enzyme inhibitor on the development of cartilage structural changes in an experimental rabbit model of osteoarthritis(OA).Methods Twenty four New Zealand rabbits were divided into control,placebo and fosinopril groups each consisted of 8 rabits.OA was surgically induced in rabbits by sectioning the anterior cruciate ligament(ACLT).OA rabbits were randomly divided into two groups and treated orally with 1)placebo,10mg/kg/day of Vitamine C;2)0.5mg/kg/day of fosinopril,the normal rabbits were taken as control.All rabbits were killed2 months after surgery.The severity of the lesions was recorded by macroscopical and histological examination.Cartilage specimens from the femoral condyles were processed by immunohistochemistry to evaluate the levels of iNOS,MMP-1,MMP-9;and quantitative reverse transcription-polymerase chain reaction(RT-PCR)were used to study the messenger RNA levels of iNOS.Results Treatment with fosinopril reduced the development of cartilage lesions(P0.01).Immunohistochemical analyses showed that treatment with fosinopril significantly reduced the synthesis of some key OA mediators:MMP-1、MMP-9(P0.05).Quantitative RT-PCR showed that fosinopril reduced the expression of iNOS in OA cartilage,but without statistics difference.Conclusion Fosinopril can reduce the progress of cartilage structural changes in a rabbit model of OA.The effect is associated with the inhibition of the major pathophysiologic mediators responsible for cartilage degradation.