I. Richter, K. Mukherjee, N. Weber
Oct 1, 1978
Citations
0
Influential Citations
4
Citations
Journal
Zeitschrift für Naturforschung C
Abstract
Abstract Simple methods are described for the preparation of trans-[1-14C]monounsaturated fatty acids and cis -[1-14C]monounsaturated fatty acids, each with a fairly uniform distribution of positional isomers, which can serve as mixed substrates in biochemical studies. Methyl trans- [1-14C] octadecenoates and methyl trans-[1-14C] eicosenoates are prepared by partial catalytic hydrogenation of methyl [1-14C] linolenate and methyl [1-14C] arachidonate, respectively, follow ed by argentation chromatography of the partially hydrogenated products. Methyl trans-[1-14C] octadecenoates and methyl trans-[1-14C] eicosenoates, thus obtained, yield the corresponding methyl cis-[1-14C] octadecenoates and methyl cis-[1-14C] eicosenoates, respectively, after trans-cis equilibration and argentation chromatography. Hydrolysis of each of these methyl esters yields the fatty acids.