G. A. Scarborough
Apr 10, 1970
Citations
3
Influential Citations
117
Citations
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Journal
The Journal of biological chemistry
Abstract
Abstract The transport of several sugars by cells of Neurospora crassa grown in shake culture has been investigated. 3-O-Methyl-d-glucose is a substrate for the glucose transport system in glucose-grown cells but cannot be phosphorylated by the ATP-dependent hexokinase which readily catalyzes the phosphorylation of d-glucose. Since this nonmetabolizable glucose analogue cannot be accumulated against a concentration gradient in cells grown on glucose, it appears that net glucose uptake in glucose-grown cells involves a facilitated diffusion transport step followed by ATP-dependent phosphorylation and subsequent metabolism of the transported glucose. Growth of cells on d-fructose leads to the appearance of a system which catalyzes the accumulation of unaltered 3-O-methyl-d-glucose and l-sorbose against considerable concentration gradients.