J. Antoine, S. Avrameas
Jul 1, 1974
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0
Influential Citations
23
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Quality indicators
Journal
European Journal of Immunology
Abstract
By using antibody‐peroxidase conjugates, quantitative data were obtained concerning events following interactions between anti‐Ig antibodies and surface Ig. About 5 × 105 molecules of peroxidase‐labeled anti‐rat Ig antibodies were bound per positive lymph node cell, at conjugate excess, at 4 °C. Similar quantities of anti‐Fcγ or anti‐Fab were bound per positive cell, suggesting that cell surface IgG antigenic determinants are largely exposed. Experiments performed on thymocytes showed that the number of peroxidase molecules bound per 106 cells was 12 to 16 times higher with lymph node lymphocytes than with thymocytes.