Leyu Wang, Lun Wang, Chang-qing Zhu
Jan 12, 2002
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0
Influential Citations
10
Citations
Journal
Analytical Letters
Abstract
ABSTRACT For the first time, 4-aminophenol was used to determine protein with a synchronous fluorescence technique. The interactions of 4-aminophenol with protein give strong signals of synchronous fluorescence at 292 nm. Based on this reaction, a new quantitative determination method for protein in aqueous solutions has been developed. At pH 6.0, with 3.0 × 10−5 mol L−1 of 4-aminophenol, linear relationships were found between the enhanced intensity of synchronous fluorescence at 292 nm and the concentration of protein in the range 0.05–4.5 µg mL−1 for human serum albumin (HSA), 0.1–2.0 µg mL−1 for bovine serum albumin (BSA) and 0.05–5.5 µg mL−1 for γ-globulin (γ-G). The limits of detection were 0.07 µg mL−1 for HSA, 0.05 µg mL−1 for γ-G and 0.08 µg mL−1 for BSA, respectively. The method has been applied to the analysis of human serum samples collected from the hospital and the results were in good agreement with those reported by the hospital, indicating that the method presented here is not only sensitive and simple, but also reliable and suitable for practical applications.