E. Gross, N. Shavit, A. San Pietro
1968
Citations
0
Influential Citations
25
Citations
Quality indicators
Journal
Archives of biochemistry and biophysics
Abstract
Abstract Synthalin, decamethylene diguanidine, has been found to act as an energy-transfer inhibitor in chloroplasts. Both ATP formation and the accompanying coupled electron flow were inhibited in a parallel manner, but neither the basal rate of electron flow (minus phosphate) nor that observed in the presence of uncouplers such as CCCP or NH 4 Cl were significantly affected. Electron flow stimulated by arsenate was inhibited to the same extent as that stimulated by phosphate. Light-induced proton uptake was not affected at synthalin concentrations which completely inhibited ATP formation. PMS mediated phosphorylation was less sensitive to synthalin than phosphorylation mediated by ferricyanide or methyl viologen since 10 −3 m synthalin was required for 50% inhibition with PMS whereas only 2 × 10 −4 m was required for 50% inhibition with the other electron transport agents.