T. Oe, M. Ikuta, T. Toyo’oka
Feb 1, 2002
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Journal
Analytical Sciences
Abstract
4-(3,17 β -Dihydroxyestra-1,3,5(10)-trien-6 α - and 6 β -yl)amino-7-nitro-2,1,3-benzoxadiazoles have been synthesized and characterized as fluorescent probes for use in a receptor assay and/or a homogeneous immunoassay for estradiol. The fluorescence intensities are strongly dependent upon the solvent polarity used. The intensities in water were reduced to less than 1% of those in ethyl acetate, and a blue shift was also observed in polar solvents. The quenched fluorescence in aqueous solution was recovered by adding bovine serum albumin or an anti-estradiol antibody. Adding intact estradiol inhibited the fluorescence recovered by the antibody.