C. Halldin, B. Långström
Jul 1, 1986
Citations
0
Influential Citations
16
Citations
Journal
Journal of Labelled Compounds and Radiopharmaceuticals
Abstract
The synthesis of [3-11C]phenylpyruvic acid, starting with 11CO2, is reported. This α-keto acid was prepared via a condensation reaction, using [11C]benzaldehyde (prepared as described elsewhere), and 2-phenyl-5-oxazolone, using diazabicyclooctane (DABCO) as a base. The condensation product, [α-11C]-4-benzylidene-2-phenyl-5-oxazolone, was converted by basic hydrolysis to give [3-11C]phenylpyruvic acid in 40 % radiochemical yield, starting with 11CO2, within 40 min. The potential of [3-11C]phenylpyruvic acid in enzymatic transamination, catalyzed by glutamic/oxaloacetic acid transaminase (GOT) (Ee 2.6.1.1) immobilized on CNBr-activated Sepharose was also studied.