R. Myers, R. Lee, Y. Lee
1980
Citations
1
Influential Citations
144
Citations
Quality indicators
Journal
Analytical biochemistry
Abstract
Abstract 4-Methylumbelliferyl α-ketoside of N-acetylneuraminic acid was synthesized by reacting the sodium salt of 4-methylumbelliferone with the 2-chloro-2-deoxy derivative of peracetylated methyl N-acetylneuraminate, followed by preparative silica gel chromatography, deblocking, and purification by gel filtration on Sephadex G-25. The final product was isolated as either the sodium or ammonium salt, and its suitability as a substrate for neuraminidase was evaluated. The optimal pH values for various neuraminidases were 5.6 in acetate buffer (Arthrobacter ureafaciens), 5.0–5.1 in acetate buffer (Clostridium perfringens), and 4.4 in phosphate-citrate buffer (human fibroblasts). Km values for these enzymes at the optimal pH were 6 × 10−4 m (Arthrobacter), 1 × 10−4 m (Clostridium), and 3 × 10−4 m (human fibroblasts).