H. Ping-fang
2012
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Journal
Journal of Chemical Engineering of Chinese Universities
Abstract
The synthesis of 4-nitrobenzyl 2-chloroacetate by using the 4-nitrobenzyl alcohol and chloroacetic acid as raw materials and immobilized lipase Novozym 435 as catalyst was investigated.The reaction conditions of enzymatic synthesis were also optimized,and the optimum conditions found are as follows: mole ratio of 4-nitrobenzyl alcohol and chloroacetic acid is 1:2,concentration of 4-nitrobenzyl alcohol is 5 g L 1,Novozym 435 concentration is 3.4 g L 1,reaction temperature is 50℃ and reaction time is 10 h.Under above optimum conditions,the yield of 4-nitrobenzyl 2-chloroacetate can reach 76.7%.In addition,the kinetics of lipase catalytic synthesis of 4-nitrobenzyl 2-chloroacetate was explored,and it was found that the kinetics of the reaction agrees with the double-substrate ping-pong procedure mechanism and acid-substrate inhibition kinetic model.Finally,the kinetic equation of the model was given.