K. Imai, H. Reeves, R. Rabin
Feb 3, 1964
Citations
0
Influential Citations
3
Citations
Journal
Biochimica et Biophysica Acta
Abstract
Abstract In experiments concerning the enzymic synthesis and breakdown of n -propylmalate by extracts obtained from aerobically-grown, valerate-adapted Escherichia coli , the following observations have been made: 1. 1. The use of [1- 14 C]glyoxylate and n -valeryl-CoA or glyoxylate and [1- 14 C] n -valeryl-CoA results in the formation of labelled n -propylmalic acid. 2. 2. When the enzymic reaction mixture is incubated for long periods of time at pH 7, oxaloacetic acid is also produced. 3. 3. The oxaloacetic acid produced is labelled when the reaction mixture contains [1- 14 C]glyoxylate, but is not labelled when [1- 14 C] n -valeryl-CoA is used. 4. 4. The oxaloacetic acid thus produced has been identified as the cis -enol form of this compound. 5. 5. Oxaloacetic acid is produced from n -propymalate during prolonged incubation periods. 6. 6. From the distribution of radioactivity, it has been postulated that, although the metabolic breakdown of n -propylmalic acid involves a complex pathway, it does not arise from a split of the side chain. 7. 7. Both ATP and CoA are required for the formation of oxaloacetate from n -propylmalic acid.