K. Nagira, M. Shiga, K. Sasamoto
Aug 23, 1994
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Journal
Bioscience, Biotechnology, and Biochemistry
Abstract
New bis-piperazine-type pH buffer agents were synthesized and their buffering properties were evaluated. The compounds proved to have two-fold larger pH buffering ability than 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES), a Good’s buffer traditionally used to control the pH value of culture media.Human-human hybridoma HB4C5 cells were cultured in a serum-free medium containing these buffer agents. The cell growth and antibody production, using 1,2-N,N′-bis[N′′,N′′′-di(2-sulfonoethyl)piperazinyl]ethane, were greater than when HEPES.