Z. Jie
2008
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Journal
Fine chemicals
Abstract
Both enantiomers of ethyl 2-hydroxy-4-phenylbutanoate(EHPB) were prepared by enzymatic reduction of ethyl 2-oxo-4-phenylbutanoate(EOPB) with two whole cells in present work.Two strains capable to reduce the ketone to the corresponding chiral alcohols were screened.The reaction conditions were optimized.The R-enantiomer was obtained in 74.5% yield with 97% e.e.under the catalysis of 25 mmol/L substrate by strain Bacillus pumilus Phe-C3;the S-enantiomer was achieved in 71.7% yield with 95% e.e.under the catalysis of 15 mmol/L substrate by strain Klebsiella pneumomiae Phe-E4.