Long-xuan Zhao, Eun-kyung Kim, Hyun-Tae Lim
Feb 1, 2002
Citations
1
Influential Citations
20
Citations
Quality indicators
Journal
Archives of Pharmacal Research
Abstract
Recently, we have reported that 2-bromopropane might have an immunotoxic potential in rats when exposed for 28 days. In the present studies, the possibility of 2í-deoxyguanosine adduct formation by 2-bromopropane was investigatedin vitro to elucidate molecular mechanism of 2-bromopropane-induced immunosuppression.N7-Guanine adduct of 2′-bromopropane (i.e.,N7-isopropyl guanine) was chemically synthesized and structurally characterized by analysis of UV,1H-NMR,13C-NMR, COSY and fast atom bombardment mass spectrometry to use as a reference material. Incubation of 2′-deoxyguanosine with an excess amount of 2-bromopropane in PBS buffer solution, pH 7.4, at 37°C for 16 h, followed by a thermal hydrolysis, produced a detectable amount ofN7-isopropyl guanine by an HPLC and UV analysis. The present results suggest that 2-bromopropane might form a DNA adduct inN7 position of 2′-deoxyguanosine at a physiological condition.