Cao Xj, Xie Wb, Panhuysen Lh
2012
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Journal
Chemical Journal of Chinese Universities-chinese
Abstract
In order to obtain a new solid phase time-resolved fluorescence immunoassay chelate, a new intermediate compound 4,4'-(p-aminophenylethynyl)-6,6'-bis[N,N-bis(ethoxycarbonylmethyl) amino methyl]-2, 2'-bipyridine was prepared from 4,4'-dinitro-2,2'-bipyridine-6,6'-dimethyl bis(trifluoroacetate) by hydrolyzation, bromination, esterification and substitution. The structure of the compound was charactered by DSC, IR, H-1 NMR and HRMS. The compound was hydrolyzed in the solution of europium ion, forming a rare earth fluorescence chelate. The fluorescence spectrum of the chelate excited by an light source has peaks peculiar to the europium ion.