D. Hwang, M. Scott
Sep 1, 1993
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0
Influential Citations
4
Citations
Journal
Bioorganic Chemistry
Abstract
Abstract The synthesis and characterization of 2-chloro-4-nitrophenyl β-D-galactopyranoside, an improved chromogenic substrate for β-galactosidase, is described. The important kinetic parameters ( K m , V max and K p ) for this substrate were compared with those of other substrates. The diagnostic utility of this substrate in a digoxin liposome immunoassay is discussed. The new substrate offers at least four times the sensitivity enhancement as that with ortho -nitrophenyl β-D-galactopyranoside in the assays for β-galactosidase. This substrate should find use in enzyme immunoassays where β-galactosidase is used as a label.