A. Vetere, S. Ferro, M. Bosco
Aug 1, 1997
Citations
1
Influential Citations
11
Citations
Journal
FEBS Journal
Abstract
P-D-Xylopyranosides, such as p-nitrophenyl-P-D-xylopyranoside (Xyl-Np) or 4-methylumbelliferyl-PD-xylopyranoside (Xyl-MeUmb), when added to the culture medium of human skin fibroblasts have previously been shown to produce some Np- or MeUmb-oligosaccharides related to the regulation of glycosaminoglycan biosynthesis. Among these oligosaccharide derivatives, we synthesized the trisaccharide derivative NeuAc(a2- 3)Gal(P1-4)Xyl-Np@l- as a potential inhibitor of human skin fibroblast glycosaminoglycan biosynthesis. This synthesis was achieved by sequential use of transglycosylating activities of Escherichia coli P-galactosidase and Tvpanosornu cruzi trans-sialidase. The structure of the oligosaccharide obtained was determined by HPLC, ion-spray mass spectrometry, and NMR.