M. Gerlach, Nicola Schwelle, W. Lerbs
1985
Citations
0
Influential Citations
29
Citations
Journal
Phytochemistry
Abstract
Abstract Cyclopeptine, a benzodiazepine alkaloid of Penicillium cyclopium, is formed from anthranilic acid, L -phenylalanine and the methyl group of L -methionine by cyclopeptine synthetase. The following partial activities of this enzyme system were determined in vitro : anthranilic acid and L -phenylalanine adenylyltransferase activity, binding of anthranilic acid and L -phenylalanine as thioesters to proteins, formation of thioester-bound N -methyl- L -phenylalanine and N -methyl- L -phenylalanylanthranilic acid. The obtained results indicate that cyclopeptine is formed via enzyme-bound intermediates by the thiotemplate mechanism of peptide biosynthesis.