Hailang Chen, Xiaofeng Bao, Shu Hai
Apr 1, 2017
Citations
0
Influential Citations
35
Citations
Journal
Sensors and Actuators B-chemical
Abstract
Abstract A new rhodamine B derivative, namely, N1-(benzo[d]thiazol-2-yl)-N4-(2-(3′,6′-bis (diethylamino)-3-oxospiro[isoindoline-1,9′-xanthen]-2-yl)ethyl)malea-mide (RDBSF), was designed, synthesized, and structurally characterized to develop a new chemosensor. UV–vis absorption and fluorescence spectroscopic studies show that RDBSF exhibits high sensitivity and selectivity toward Fe3+ in the presence of many other metal cations in a Tris-HCl solution (1 mM, pH = 7.4) containing 30% MeCN by forming a 1:1 complex with Fe3+. The binding association constant (Ka) of RDBSF for Fe3+ was estimated as 1.52 × 104 M−1 in the solution. The detection limit of Fe3+ by RDBSF was further determined as 11.6 nM. Intracellular imaging applications demonstrated that RDBSF can be used as a fluorescent probe for the detection of Fe3+ in HepG-2 cells.