K. Chilvers, Arthur James, John Perry
Dec 1, 2001
Citations
0
Influential Citations
49
Citations
Journal
Journal of Applied Microbiology
Abstract
Aims: A widely used coumarin derivative is 7‐hydroxy‐4‐methylcoumarin‐β‐D‐galactoside (4‐methylumbelliferone‐β‐D‐galactoside; 4‐MU‐GAL). This galactoside is utilized as a substrate for the detection of the β‐galactosidase activity of coliform bacteria in water analysis. The intense fluorescence of coumarin‐based molecules has enabled them to be incorporated into enzyme‐based tests for the quantitative assay of indicator bacteria. The aim of this present study was to evaluate the potential of other coumarin derivatives, by synthesis of a selection of core coumarin molecules.