Kazuhiro Watanabe, R. Kimura, I. Yoshizawa
May 25, 1986
Citations
0
Influential Citations
2
Citations
Journal
Chemical & Pharmaceutical Bulletin
Abstract
For studies on the enzymatic O-methylation of 3, 4-dihydroxyestra-1, 3, 5(10)-trien-17β-ol 17-sulfate (Ie), its two monomethyl ethers, 4-hydroxy-3-methoxyestra-1, 3, 5(10)-trien-17β-ol 17-sulfate (IId) and 3-hydroxy-4-methoxyestra-1, 3, 5(10)-trien-17β-ol 17-sulfate (IIId), were prepared as authentic specimens.An assay procedure for these guaiasols including the isomeric sulfates, 3-hydroxy-2-methoxyestra-1, 3, 5(10)-trien-17β-ol 17-sulfate (Ic) and 2-hydroxy-3-methoxyestra-1, 3, 5(10)-trien-17β-ol 17-sulfate (Id), was established by using reversed-phase high-performance liquid chromatography. The devised technique involves elution with a mixture of 0.5% NH4H2PO4 (pH 3.0) and methanol (55 : 45, v/v) on a TSK gel ODS-120A column and monitoring with an electrochemical detector.