Zhu Guo
2002
Citations
0
Influential Citations
2
Citations
Journal
Journal of Zhejiang Agricultural University
Abstract
This study reported that the first compound, 2,3 dihydro 2,2 dimethyl 7 benfuranyl chloroformate (DDBC) was synthesized after using 2,3 dihydro 2,2 dimrthyl 7 benzofuranol reacted with CoCl 2. Then two haptens named 4 [〔(2,3 dihydro 2,2 dimethyl 7 benfuranyloxy) carbonyl〕 amino] hexanoic acid(BFNH) and 6 [〔2,3 dihydro 2,2 dimethyl 7 benfuranyloxy) carbonyl〕 amino] butanoic acid(BFNB) were synthesized with the reaction between DDBC and amino hexanoic acid or amino butanoic acid under different conditions. The BFNH and BFNB were conjugated to bovine serum albumin (BSA) with the modified active ester method to form two antigens for producing the high titre antibodies to carbofuran. The ratios of BFNH BSA and BFNB BSA were 10∶1 and 13∶1, respectively. Other two coating antigens for carbofuran, BFNH OVA and BNFB OVA were also synthesized by conjugating BFNH or BFNB to ovalbumin (OVA) with the mixed anhydride method, and their ratios were 4∶1 and 3∶1, respectively.