H. Lee, Songhee Kim, E. Kim
Jun 28, 2019
Citations
2
Influential Citations
14
Citations
Journal
Journal of microbiology and biotechnology
Abstract
Anthranilate derivates are used as flavoring and fragrant agents for a long period time. Recently, these compounds gain an attention due to the new biological functions including antinociceptive and analgesic activities. Three anthranilate derivatives, N-methylanthranilate, methyl anthrannilate, and methyl N-methylanthranilate were synthesized using metabolically engineered stains of Escherichia coli. NMT encoding N-methyltransferase from Ruta graveolens, AMAT encoding anthraniloyl-coenzyme A (CoA):methanol acyltransferase from Vitis labrusca, and PqsA encoding anthranilate Coenzyme A ligase from Pseudomonas aeruginosa were cloned and E. coli strains harboring these genes were used to synthesize the three desired compounds. E. coli mutants (metJ, trpD, tyrR mutants), which provide more anthranilate and/or S-adenosyl methionine, were used to increase the production of the synthesized compounds. MS/MS analysis was used to determine the structure of the products. Approximately, 185.3 μM N-methylanthranilate and 95.2 μM methyl N-methyl anthranilate were synthesized. This is the first report about the synthesis of anthranilate derivatives in E. coli.