Wang Xiaosa
2013
Citations
0
Influential Citations
0
Citations
Journal
China Oils and Fats
Abstract
The improved synthesis of monoolein by twostep method was reported.First,1,2-acetonide-3-oleoylglycerol was synthesized by the enzymatic esterification of 1,2-acetonide glycerol and oleic acid,then monooleic was synthesized by cleavage of 1,2-acetonide-3-oleoylglycerol in methanol catalysted by Amberlyst-15.The effects of solvent addition amount,enzyme dosage,reaction temperature and reaction time on the 1,2-acetonide-3-oleoylglycerol content in the crude product were studied.The results showed that the optimal conditions were that oleic acid and 1,2-actetonide glycerol(molar ratio 1:1)reacted in 0.5 mL dichloromethane at 60 ℃ for 6 h with 8% Novozym 435(based on the total masses of substrates)as catalyst.Under the optimal conditions,the 1,2-acetonide-3-oleoylglycerol content was 91.7%.Crude monoolein was synthesized by cleavage of unpurified 1,2-acetonide-3-oleoylglycerol in methanol with Amberlyst-15 resin as catalyst,then purified by hexane recrystallization and washing,and the monoolein content increased from 72.6% to 95.8%.