F. Hayes, E. Hansbury, V. Mitchell
Dec 1, 1968
Citations
0
Influential Citations
12
Citations
Journal
European journal of biochemistry
Abstract
The N-acetylated deoxyadenosine and deoxyguanosine 5′-triphosphates (dAAcTP and dGAcTP) have been synthesized and found competent as substrates for enzymatic chain lengthening of polydeoxyribonucleotides without concomitant loss of the acetyl group. The enzyme used was terminal deoxyribonucleotidyltransferase. The addition of dGAc units proceeds without aggregation that self-limits addition of nonacetylated dG units. The resulting polymer 3′-ended with dGAc units is an efficient initiator for subsequent chain lengthening. Hydrogen bonding is not destroyed by N-acetylation of the dA-dT Watson-Crick pair, although it is considerably weakened. As a practical result of this, a polymer 3′-ended with dAAc units is a much more efficient initiator for enzymatic chain lengthening by dT units than is a corresponding polymer with dA units. Venom phosphodiesterase does not hydrolyze a polymer 3′-ended with dAAc units.