K. Skowera, M. Kańska
Jul 1, 2008
Citations
0
Influential Citations
5
Citations
Journal
Journal of Labelled Compounds and Radiopharmaceuticals
Abstract
The synthesis of isotopomers of phenylpyruvic acid, PPA, selectively labeled with hydrogen isotopes in the 3-position of the side-chain is reported. Three deuterium or tritium labeled isotopomers of L-phenylalanine, L-Phe, i.e. [(3S)-2H]-L-, [(3S)-3H]-L-, and doubly labeled [(3S)-2H/3H]-L-Phe were synthesized using the enzyme phenylalanine ammonia lyase (EC 4.3.1.5). In the second step these isotopomers of L-Phe were converted into [(3S)-2H], [(3S)-3H]-, and [(3S)-2H/3H]-isotopomers of PPA using the enzyme L-phenylalanine dedydrogenase (EC 1.4.1.20). The isotopomer of PPA labeled with 14C in carboxylic group, [1-14C]-PPA, was obtained in a two-step enzymatic reaction using [1-14C]-cinnamic acid as the starting substrate. Copyright © 2008 John Wiley & Sons, Ltd.